"The latest tests on the farm to the east of the surveillance zone are negative," Chief Veterinary Officer Debby Reynolds said.
Reynolds announced late Thursday that cows in a second area of the southern England county of Surrey had shown "mild clinical signs of infections" and were being tested. Officials established an exclusion zone around a farm previously unlinked to the outbreak and some 10 miles from the two confirmed cases of the disease.
The Department for Environment, Food and Rural Affairs on Saturday lifted restrictions around the farm, near the village of Wotton.
British authorities are increasingly confident that they have prevented foot-and-mouth from spreading to livestock across the country, a development that could have devastated the rural economy. An epidemic in 2001 led to the slaughter of 7 million animals and shut British meat out of world markets for months.
Prime Minister Gordon Brown said Friday that officials had "restricted the disease to a limited area of this country."
"The risk of it spreading out of these areas is low, if not negligible," he said.
An interim epidemiology report on the outbreak has concluded that the disease probably spread by human movement from a research laboratory facility in Pirbright, about 30 miles southwest of London.
The complex houses vaccine-maker Merial Animal Health - the British arm of U.S.-French pharmaceutical firm Merial Ltd.
- and the government's Institute of Animal Health.