The Flyers' remarkable turnaround from the worst team in the NHL last season to the Eastern Conference playoffs was completed when Florida beat Carolina 4-3 shortly after Philadelphia's win.
R.J. Umberger scored his 13th goal of the season only 5:44 into the game, and Scottie Upshall and Joffrey Lupul scored 37 seconds apart in the third.
Biron, who has never played a postseason game, recorded his 22nd career shutout.
There could be a Philly playoff double on Friday night. The 76ers needed to win at Atlanta to reach the postseason for the first time since 2005.
Martin Brodeur made 33 saves and was having one of the more dazzling performances against the Flyers by a visiting goalie this season until the third. The Flyers attacked Brodeur all three periods, putting him on his back, on his knees or sprawled along the crease on almost every shot. He stopped just about everything until he allowed two straight goals and was pulled for Kevin Weekes.
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The Flyers played without Danny Briere, who was scratched because in injured left knee. Briere, who has 31 goals and 72 points, injured his knee in a collision during Wednesday's 4-2 loss in Pittsburgh.
"I want to help out the team," Briere said. "We all are aware of the situation. But sometimes there is just nothing you can do."
Trying to win their first Stanley Cup since 1975, the Flyers rebounded from the most disastrous season in franchise history. They won only 22 games and missed the playoffs for the first time since 1994, forcing general manager to overhaul the roster and add key players like Briere, Lupul and defensemen Jason Smith and Kimmo Timonen.
General manager Paul Holmgren set the preseason goal of playing meaningful games in March. Now, the Flyers will play big ones in April and possibly beyond.