The agency sent letters this week to more than a dozen Web-based companies saying they are violating a new ban and asking the companies to describe in writing what action they have taken to comply.
The FDA banned candy-, fruit- and clove-flavored cigarettes in September. Federal health authorities and regulators say those products appeal especially to young people and are thought to attract new smokers.
"FDA takes the enforcement of this flavored cigarette ban seriously," Dr. Lawrence R. Deyton, director of FDA's Center for Tobacco Products, said in a statement. "These actions should send a clear message to those who continue to break the law that FDA will take necessary actions to protect our children from initiating tobacco use."
Citing research, the FDA has said that 17-year-old smokers are three times as likely to use flavored cigarettes as smokers over the age of 25.
Almost 90 percent of adult smokers picked up the habit as teenagers, the agency has said, and the ban will help prevent an average of more than 3,600 young people each day from starting smoking.
The ban on manufacturing, importing, marketing and distributing flavored cigarettes does not include menthol cigarettes or some flavored tobacco products like cigars. The FDA is studying those products.