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Armstrong's attorneys say they believe USADA investigators coerced false testimony from witnesses by promising not to charge them with doping; they argue this could violate bribery laws.
They also question whether USADA improperly gained access to testimony in a recent federal grand jury criminal investigation that ended in February with no charges filed against Armstrong.
The letter notes that Tygart participated in witness interviews with federal criminal investigators.
Armstrong's letter also challenged the 2009-2010 blood tests, which were taken during his two-year comeback from retirement. Armstrong passed all his drug tests during that period and posted his testing results on his website, Livestrong.com, and no charges were brought, the letter said.
It also said most of the allegations fall outside of USADA's eight-year statute of limitations but the agency argues that Armstrong keeps expanding the time limit by continuing to deny drug use.
USADA has said it is also pursuing doping charges against Johan Bruyneel, the manager of Armstrong's winning Tour de France teams; team doctors Pedro Celaya and Luis Garcia del Moral; team trainer Pepe Marti, and consulting doctor Michele Ferrari.
Bruyneel, who now leads the RadioShack-Nissan-Trek team, said Friday he won't attend this year's Tour de France because the allegations would be a distraction for his team and the race. Bruyneel says he is innocent.
Copyright 2012 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.
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