The exhibit, called "The Guitar: An American Love Story," opens Thursday.
Among the instruments on loan for the show are a guitar played by Elvis Presley, Eric Clapton's 1958 Gibson Explorer and singing cowboy star Roy Rogers' OM-45 Deluxe guitar made by C.F. Martin & Co., circa 1930. There will be more than 150 guitars in all.
A 150-page catalog about the collection is on sale in the museum bookstore, and there are viewing stations where visitors can see and hear the instruments being played by great musicians.
There is no admission charge at the museum in the Polk Cultural Center near the state Capitol in Nashville.