The Florida Highway Patrol said the accident occurred at about 6:45 p.m. Friday on SR 33 in Lake County. Police said in a news release that "for an unknown reason" the driver lost control of his 2009 Mercedes, which struck several trees and a pole before hitting another tree and coming to a rest.
Police said the ages of the children haven't been confirmed. The 41-year-old male driver and a male child were pronounced dead at the scene; a female toddler and a male child died at Southlake Hospital.
The driver and the children were wearing seatbelts or child restraints.
The crash remains under investigation.
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