Surveillance footage from Jan. 29 shows Hillsborough County deputy Charlette Marshall-Jones, 44, dumping Brian Sterner out of his wheelchair and searching him on the floor after he was brought in on a warrant after a traffic violation.
Sterner, 32, said when he was taken into a booking room and told to stand up, Jones grew agitated when he told her that he could not.
Marshall-Jones was suspended without pay, and three other deputies were placed on administrative leave pending an investigation.
Marshall-Jones is charged with abuse of a disabled person, a third-degree felony, said Hillsborough County Sheriff David Gee.
If convicted, she could be sent to prison for five years.
Gee said Marshall-Jones was aware of the warrant for her arrest, but that he didn't know when she might turn herself in.
Marshall-Jones could not be reached by phone for comment Friday night. A telephone number listed in her name has been disconnected.
Sterner, who can drive a car but has not been able to walk since a 1994 wrestling accident, was arrested at his Riverview home and taken to the Orient Road Jail on a charge of fleeing and attempting to elude a police officer, according to records. He had called for charges to be filed against Marshall-Jones.