Knox was convicted last year of sexually assaulting and murdering her British roommate, Meredith Kercher, and sentenced to 26 years in prison. Her co-defendant and ex-boyfriend, Raffaele Sollecito of Italy, was convicted of the same charges and sentenced to 25 years.
Both deny wrongdoing in the 2007 slaying.
The defense maintains that DNA traces presented at the original trial were inconclusive, and also challenged that they may have been contaminated when they were analyzed.
Prosecutor Giancarlo Costagliola said a review would be "useless" and that "this court has all the elements to be able to come to a decision."
Knox, a 23-year-old from Seattle, was hunched and pale as she was escorted into the courtroom. Her mother, Edda Mellas, was in court and said she would stay to spend Christmas with her daughter.
The hearing was held just two days after Italy's highest criminal court upheld the conviction and 16-year-prison sentence of the third person charged with the murder, Rudy Hermann Guede of the Ivory Coast. Guede has admitted being at the house the night of the murder but denies killing Kercher.
He has been tried separately.
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