Edinburgh Zoo had hoped the pair might mate after two earlier failures but found Thursday that the female's hormone levels had dropped, closing a brief reproductive window.
Zookeepers say the male Yang Guang and female Tian Tian showed signs of attraction to each other, but did not mate.
The 8-year-old animals met twice Wednesday out of public view, as keepers attempted to create a romantic atmosphere.
Video released by the zoo showed the pandas grappling playfully, but no more.
The animals are on loan from China, and the first pandas to live in Britain in nearly two decades. Their names mean Sunshine and Sweetie.
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