Ayman al-Zawahri called on Sunnis everywhere to devote their lives, money and expertise for the fight and prevent a U.S.-allied government from taking over in post-Assad Syria.
He urged Sunnis to "rise above their differences" and fight expanding Shiite influence in Syria.
The message came in a new audio recording posted on Thursday on the Internet. Its authenticity could not be independently confirmed but the message was posted on a militant website commonly used by al-Qaida.
Al-Zawahri has repeatedly called for holy war in Syria and has blasted Lebanese Hezbollah militants and Iran for supporting Assad.
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