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			 Theories range from an increase in construction runoff to 
			inclement weather to people clearing mangroves to allow larger boats 
			into the area. 
 			"We have been compiling data," Carmen Guerrero, secretary of the 
			Department of Natural Resources, said in a phone interview as she 
			headed toward Las Cabezas de San Juan Nature Reserve with a team of 
			scientists to investigate the problem. "There are a lot of factors 
			that could be at play."
 			The bioluminescent lagoon, often referred to as a bay, attracts 
			tourists who set out in kayaks or boats by night from the 
			neighboring city of Fajardo to see waters that glow when microscopic 
			organisms are disturbed. A greenish light swirls off hands and arms 
			as visitors trail them through the water.
 			But the bioluminescence has dropped so much that tour operators have 
			had to cancel trips and reimburse visitors, Guerrero said. 						
			
			 
 			Fajardo Mayor Anibal Melendez told reporters that the lagoon has 
			been dark for at least eight days.
 			"We've never seen anything like that," he said.
 			Melendez blamed runoff from the construction of a nearby water and 
			sewer treatment plant, and he has asked that the plant be moved 
			elsewhere.
 			Officials involved with the plant deny it's the cause. But as a 
			preventive measure, the government temporarily suspended 
			construction at the project for two weeks until scientists from 
			various agencies including the U.S. Geological Survey can figure out 
			what is causing the problem, Guerrero said.
 			She stressed that officials do not know if possible construction 
			runoff is to blame. Recent rains and a storm that is generating 
			heavy waves is another possibility for affecting the lagoon's 
			bioluminescence, Guerrero said.
 			"It's important to give these experts room so they can do their job 
			and help us understand what happened in the lagoon and why it has 
			temporarily lost some of its brightness," she said.
 			Alberto Lazaro, president of the state Water and Sewer Authority, 
			said he will evaluate scientists' findings before deciding how to 
			proceed in several weeks.
 			
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			He said the treatment plant is needed because people are discharging 
			sewage into the lagoon and nearby ocean. The plant, which is to be 
			completed by 2016, was approved by the territory's Department of 
			Natural Resources and the U.S. Environmental Protection Agency, 
			which is providing the funding.
 			"The goal of the project is to conserve this valuable resource, and 
			we have designed and developed this project taking all those 
			requirements into account," Lazaro said.
 			Laura Velez, who heads the government Environmental Quality Board 
			that monitors such projects, said that the treatment plant has had 
			adequate erosion and sediment control and that officials have 
			regularly inspected it to ensure compliance with environmental 
			standards.
 			The Conservation Trust of Puerto Rico, which manages the lagoon and 
			surrounding areas, collects water samples three times a week to 
			record data including temperature, salinity and precipitation. 
			Guerrero said scientists will analyze that data to help solve the 
			mystery of the darkening lagoon.
 			Miguel Sastre, a biology professor at the University of Puerto Rico 
			who has long studied Fajardo's bioluminescent lagoon, one of three 
			in the territory, said it went nearly dark in 2003 for unknown 
			reasons, but rebounded a couple of months later.
 			He said in a phone interview that his main concern this time is the 
			construction of the water and sewage treatment plant because it 
			coincides with the drop in bioluminescence. 			
			
			 
 			"We have to take action and evaluate the situation," Sastre said. 
			"We should do our utmost to help preserve these sites." [Associated 
					Press; DANICA COTO] Copyright 2013 The Associated 
			Press. All rights reserved. This material may not be published, 
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