Venezuela will face a surprising Netherlands team earlier in the day.
Chris Iannetta hit a two-run homer in the eighth inning but Team USA couldn't finish the comeback in the ninth.
Jimmy Rollins walked and Derek Jeter reached on a one-out error by third baseman Luis Maza. With cleanup hitter Kevin Youkilis at the plate, the United States tried a double steal and Blanco's strong throw cut down Jeter
-- the trail runner -- at second base.
Francisco Rodriguez wrapped up the save by striking out Youkilis.
Victor Zambrano allowed one run and three hits in 3 2-3 innings to earn the win before a crowd of 12,358. He walked two and struck out one.
Venezuela's bullpen was tagged for 13 runs over five inning in Sunday's rout by the U.S. but redeemed itself in this one, with five relievers combining to allow two runs in six innings of work.
Baltimore right-hander Jeremy Guthrie was the loser, giving up four runs and seven hits in two-plus innings. He walked two and struck out two.
Venezuela opened the scoring in the third when Henry Blanco, a veteran backup catcher now with the San Diego Padres, led off with a homer to left off Ted Lilly, the U.S. starter.
Team USA tied it in the fourth. Jeter singled, went to second on Adam Dunn's walk and scored on Ryan Braun's double down the first-base line.
Venezuela reclaimed the lead in the fifth against Guthrie. Henry Blanco doubled and scored on Gregor Blanco's triple to center.
Guthrie was tagged for two more in the sixth. Miguel Cabrera led off with a double, Magglio Ordonez walked and was replaced by pinch-runner Endy Chavez, and Marco Scutaro drove in both with a single.