Mahdi Karroubi's sahamnews.org says security officers are stationed at the entrance to Karroubi's house in Tehran on Thursday and are preventing relatives from meeting him.
Karroubi and Mir Hossein Mousavi have asked for permission to hold a rally on Monday in support of uprisings in Egypt and Tunisia.
State Prosecutor Gholam Hossein Mohseni Ejehi has rejected the demand and warned of repercussions if the rally takes place.
Iran crushed opposition protests against President Mahmoud Ahmadinejad's 2009 re-election. The opposition says the presidential election was rigged.
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