Knox was convicted of murdering her British roommate in Perugia, Meredith Kercher, and sentenced to 26 years in prison. Her co-defendant and ex-boyfriend Raffaele Sollecito was also convicted and sentenced to 25 years.
They both deny wrongdoing and have appealed the 2009 verdict.
The defense witnesses were called to refute testimony in the first trial from a homeless man who said he had seen the defendants near the house the night Kercher was killed, Nov. 1, 2007.
The two deny being where the homeless man had placed them.
The witnesses Saturday included people who operate buses and discos in the area.
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